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Microsatellite instability (MSI) testing

Labcorp offers Microsatellite Instability (MSI) testing to researchers for determination of the MSI status between a human tumor sample and a control sample from the same individual (human tissues and/or extracted DNA from human tissue) and/or human cell lines.

MSI Testing

Microsatellite instability [7] is a change in the length of a microsatellite allele due to insertion  or deletion of repeating units during DNA replication and failure of the  DNA mismatch repair system to correct these errors.  Alleles present in  the tumor sample but not found in the corresponding reference sample  indicates presence of microsatellite instability.  Microsatellite  Instability (MSI) analysis can be used as a screening method to identify  human tumor samples in need of further characterization (e.g. for gene  mutations involved in specific cancer pathways).

The  kit used for human MSI testing (Promega Corporation) includes  fluorescently labeled primers for co-amplification of seven markers  including five mononucleotide repeat markers (BAT-25, BAT-26, NR-21,  NR-24, and MONO-27) and two pentanucleotide repeat markers (Penta C and  Penta D).  The mononucleotide markers are used to detect MSI and the  pentanucleotide markers are used as quality control markers to detect  possible sample mix-ups and/or contamination with another sample.

Call us at 1-800-IDENTITY (USA) or 1-513-985-9777 (INTL) or Contact Us for more information and for pricing.  Volume discounts available.

Please note: This test is for in vitro research use only.  Not for use in diagnostic procedures.

Sample Preparation Instructions

We  accept cell lines (fresh, frozen, dried cell pellets), tissue or genomic  DNA from cell lines or tissue of interest (please note, extra fees may  be charged for FFPE or other difficult samples)

We  can use ONE sample for STR profiling (human and/or mouse  cell line authentication), qPCR mycoplasma testing, and MSI testing; same minimum volume/cell  requirements as for human cell line authentication and/or mouse cell line authentication.